dCasMINI Guide Design Tool


We designed a spacer design tool that allows users to retrieve genome-wide activation and suppression guides for nuclease inactivated dCasMINI. (Xu et al., 2021) This tool should facilitate epigenetic modulation studies involving dCasMINI (or other dCas molecules recognizing TTTR PAMs) by computing genome-wide activation/suppression guides that can be queried by users based on gene IDs. Compared to dCas9, dCasMINI is a fraction of the size, facilitating therapeutic delivery across both in vivo and ex vivo delivery vehicles (i.e. AAV, LNP, lentivirus/retrovirus), and has a favorable off-target profile, resulting in a reduction of undesired gene targeting events. (Xin et al., 2022)